barcode primer mix  (fluidigm)

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Identification of Open Chromatin Regions in Plant Genomes Using ATAC-Seq

doi: 10.1007/978-1-4939-7318-7_12

Figure Lengend Snippet: Transposed DNA Amplification mix

Article Snippet: Mix well, and perform PCR cycling as described in (see Note 7 ). table ft1 table-wrap mode="anchored" t5 caption a7 Component Volume (μL) Transposed DNA (from Subheading 3.3 step 4) 10 Water 10 25 μM ATAC Primer 1 2.5 25 μM ATAC barcoded Primer 2 * 2.5 2X NEBNext High Fidelity PCR Mix 25 Total 50 Open in a separate window * A different barcoded Primer 2 should be used for each library that is to be pooled into a single sequencing run.

Techniques: Amplification

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Identification of Open Chromatin Regions in Plant Genomes Using ATAC-Seq

doi: 10.1007/978-1-4939-7318-7_12

Figure Lengend Snippet: qPCR Library Amplification Mix

Article Snippet: Mix well, and perform PCR cycling as described in (see Note 7 ). table ft1 table-wrap mode="anchored" t5 caption a7 Component Volume (μL) Transposed DNA (from Subheading 3.3 step 4) 10 Water 10 25 μM ATAC Primer 1 2.5 25 μM ATAC barcoded Primer 2 * 2.5 2X NEBNext High Fidelity PCR Mix 25 Total 50 Open in a separate window * A different barcoded Primer 2 should be used for each library that is to be pooled into a single sequencing run.

Techniques: Amplification